Abstract
Therapeutic Ocular Surface Medium (TOSM) is a potential new treatment for patients
with ocular surface disorders such as dry eye and persistent epithelial defect (PED).
New therapies are needed as many patients with dry eye and PED continue to suffer
despite maximal standard therapy, and while efficacious autologous serum therapy is
not routinely available. Like serum, TOSM contains tear components and was expected
to have some of the physiological effects of tears.
Clinical and in vitro studies were used to evaluate two similar formulations of TOSM.
To comply with local pharmacy manufacturing policies, components were omitted from
TOSM v1 to produce TOSM v2. In pilot studies, conducted over 1 month, TOSM v1
improved dry eye signs and symptoms and healed over a quarter of PED. In a 2 month
randomised double-masked trial, TOSM v2 improved the signs and symptoms of dry
eye but was not superior to saline (placebo). No serious or irreversible side-effects
occurred. The altered composition of TOSM v2 may have reduced its efficacy.
However, a significant improvement in blepharitis (eyelid margin disease) and
conjunctival impression cytology (an objective measure of ocular surface health) was
found with TOSM v2. Improvement in blepharitis is an encouraging finding as it has
not been reported in other dry eye trials.
It was hypothesised that TOSM would benefit ocular surface disorders by improving
ocular surface health. In vitro, primary and cell line human corneal epithelial cells were
supported by TOSM v1 and TOSM v2. Outgrowth from limbal explants and corneal reepithelialisation
following wounding occurred with TOSM v2. This and the impression
cytology findings support our hypothesis. Further, ocular surface damage with dry eye
and PED may activate the corneal wound healing response. For wound healing,
compared to human serum, TOSM v1 and TOSM v2 had beneficial effects in vitro on
epithelial cells and human corneal fibroblasts. This may translate into a reduction in
potentially vision-threatening corneal scarring in vivo with TOSM. However, ocular
surface disorders are a heterogenous group and wound healing is a complex process
such that different preparations of TOSM may be needed for use in different disorders
and at different stages of the disease process.